New cellular component with importance for the sense of smell discovered NatureComms
In summary, our study identifies an odorant-regulated vesicular pathway in the dendrites of OSNs in which MVTs transports and stores ciliary olfactory transduction proteins and harbors constitutive proteins such as ESCRT-0, RP2, and synaptophysin. The results suggest that MVTs play an important regulatory role in the compartmentalization and sorting of GPCR signaling components in dendrites.
Tissue was dissected and incubated for 30 min in fixative in 0.1 M phosphate buffer pH 7.4. The OE from turbinates was fixed by incubation for 10 min at 20 °C in a vacuum using the PELCO BioWave® Pro+ Microwave Processing System. The tissue was then washed three times for 5 min in PBS and one time for 5 min in 1% glycine/PBS , transferred to a 1 mm thin sheet of 12% gelatin, and incubated for 30 min at 37 °C.
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