A new class of antibodies against COVID-19 is being put to the test NatureComms
SARS-CoV-2 specific memory B cells were characterized and sorted via flow cytometric tetramer assay essentially as previously described:
biotinylated SARS-CoV-2 RBD , spike and SARS-CoV-1 RBD were incubated with Streptavidin-APC , Streptavidin-PE , Streptavidin-BB515 or BV650-PE at a 4:1 molar ratio. Streptavidin conjugated fluorophores were titrated onto biotinylated antigen in 1/10Cryopreserved PBMCs from five convalescent patients were thawed and suspended in pre-warmed RPMI-1640 media containing 10% FBS , 2 mM L-glutamine, 50 IU/ml penicillin and 50 µg/ml streptomycin.
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