Engineered whitebloodcells can eliminate cancer, shows study Penn natBME
, Flow cytometry analysis of surface markers on conditionally immortalized macrophage progenitors. Staining for myeloid markers on CIM progenitors and freshly harvested bone marrow cells for comparison. Flow histograms of SIRPα staining on SIRPα KO CIM progenitors and unedited wild-type CIM progenitors. Anti-SIRPα binding was detected with AF546 anti-rat IgG., Convalescent serum IgG from CD47 KO tumour complete responders opsonizes CD47 KO, DKO, and WT B16.
Extended Data Fig. 8 Convalescent sera from CD47 KO tumour complete responders contain multiple subclasses of IgG that bind CD47 KO and DKO B16, but not syngeneic YUMM2.1 melanoma cells., Kinetics of de novo IgG generation revealed by flow cytometry of CD47 KO and DKO B16 cells stained with serum followed by a polyclonal anti-mouse IgG [H+L] secondary antibody or secondary antibodies specific for mouse IgG1 and IgG3 . The corresponding data for IgG2a/c and IgG2b are shown in Fig..
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