In a large-scale study funded by Kings College London, researchers successfully implemented an automated PCR-based testing program for SARS-CoV-2 using saliva samples from asymptomatic students and staff. The system achieved 85% sensitivity and 100% specificity, provided rapid results within eight hours, and offered a cost-effective and scalable approach to community surveillance of COVID-19.
By Pooja Toshniwal PahariaAug 28 2023 In a recent study posted to the medRxiv preprint* server, researchers established a polymerase chain reaction -based severe acute respiratory syndrome coronavirus 2 testing program in an educational institution, analyzing saliva samples obtained from asymptomatic students and staff.
*Important notice: medRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information. The exam was created following the UK Department of Health and Social Care criteria. Asymptomatic individuals were motivated to test every day . The primary outcome measures were the number of COVID-19 tests performed, the number of individuals tested, and the rate of SARS-CoV-2 positivity. The samples were processed using an automated, open source-low-cost framework using non-commercial-type reagents.
Results In total, 58,277 PCR tests were performed on saliva, with 2,989 positive , collected from 20,186 individuals. During the study period, 160,000 samples were processed using the KCL methodology. Every day for 18.0 months, between 10.0 and 30.0% of campus footfall was evaluated. To detect SARS-CoV-2, the turnaround time was eight hours, and the detection limit was 100 copies per mL.
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