New kidney MRI dye promises more accurate, personalized mapping NatureComms
voxel k values derived from each mouse. Maps of AUC were overlaid on top of the image acquired atGlucoverdazyl-enhanced scans were acquired from healthy male BALB/c mice as described in thesection, and normalized intensity-over-time curves were acquired as described in thesection, with ROIs drawn for kidney, liver, bladder, and muscle tissues.Blood was drawn from the saphenous vein at day 0 and at each post-injury time point prior to glucoverdazyl-enhanced MRI.
The pellet was resuspended in 60 µl of mobile phase and samples were quantified by HPLC, integrating the matched-time elution peak seen in the standard curve at 234 nm.Glucoverdazyl-enhanced scans for all mice in both UUO and FAN disease models were acquired as described in thesection, and normalized intensity-over-time curves were acquired as described in thesection, with ROIs drawn for kidney tissue. All studies were initiated when animals were 9–10 weeks of age.
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