Testing hundreds of therapeutics outside the body to predict their effectiveness against multiple myeloma ETH_en naturecancer
cells; selection of any plasma cell-marker-positive cells ; and separation by size into ‘big’ and ‘small’ plasma-positive cells using FSC and SSC gates. Cells were analyzed using BD FACSDIVA software and FlowJo software.
cells per ml in 5% FBS medium. Then, 200 µl suspension was pipetted into the cytofunnel and slides were centrifuged at 450 r.p.m. for 5 min. The slides were removed from the centrifuge and fixed for 15 min at 4 °C in 70% ethanol . MS/MS spectra assignment was performed with Proteome Discoverer 2.1 using Sequest HT and MS Amanda as search nodes together with Percolator. For spectra annotation, a UniProt database filtered for, concatenated with a common contaminant and a standard peptide.fasta file was used. The following search parameters were used for protein identification: peptide mass tolerance set to 10 ppm; MS/MS mass tolerance set to 0.
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