In the age of at-home testing, we have a myriad of diagnostic tools at our fingertips. But how much can we find out about our health and fitness from our DNA? Strong Women’s Lauren Geall gives one test a go.
Belgique Dernières Nouvelles, Belgique Actualités
Similar News:Vous pouvez également lire des articles d'actualité similaires à celui-ci que nous avons collectés auprès d'autres sources d'information.
Reversal of dual epigenetic repression of non-canonical Wnt-5a normalises diabetic corneal epithelial wound healing and stem cells - DiabetologiaAims/hypothesis Diabetes is associated with epigenetic modifications including DNA methylation and miRNA changes. Diabetic complications in the cornea can cause persistent epithelial defects and impaired wound healing due to limbal epithelial stem cell (LESC) dysfunction. In this study, we aimed to uncover epigenetic alterations in diabetic vs non-diabetic human limbal epithelial cells (LEC) enriched in LESC and identify new diabetic markers that can be targeted for therapy to normalise corneal epithelial wound healing and stem cell expression. Methods Human LEC were isolated, or organ-cultured corneas were obtained, from autopsy eyes from non-diabetic (59.87±20.89 years) and diabetic (71.93±9.29 years) donors. The groups were not statistically different in age. DNA was extracted from LEC for methylation analysis using Illumina Infinium 850K MethylationEPIC BeadChip and protein was extracted for Wnt phospho array analysis. Wound healing was studied using a scratch assay in LEC or 1-heptanol wounds in organ-cultured corneas. Organ-cultured corneas and LEC were transfected with WNT5A siRNA, miR-203a mimic or miR-203a inhibitor or were treated with recombinant Wnt-5a (200 ng/ml), DNA methylation inhibitor zebularine (1–20 µmol/l) or biodegradable nanobioconjugates (NBCs) based on polymalic acid scaffold containing antisense oligonucleotide (AON) to miR-203a or a control scrambled AON (15–20 µmol/l). Results There was significant differential DNA methylation between diabetic and non-diabetic LEC. WNT5A promoter was hypermethylated in diabetic LEC accompanied with markedly decreased Wnt-5a protein. Treatment of diabetic LEC and organ-cultured corneas with exogenous Wnt-5a accelerated wound healing by 1.4-fold (p|0.05) and 37% (p|0.05), respectively, and increased LESC and diabetic marker expression. Wnt-5a treatment in diabetic LEC increased the phosphorylation of members of the Ca2+-dependent non-canonical pathway (phospholipase Cγ1 and protein kinase Cβ; by 1.15-fold [
Lire la suite »
Man who killed NH beauty school student Laura Kempton in 1981 is identified: WATCH LIVEThe man who killed a beauty school student in Portsmouth, New Hampshire, more than four decades ago has been identified, authorities said Thursday. Laura Kempton, 23, was found dead of massive head trauma at an apartment on Chapel Street on Monday, Sept. 28, 1981, according to an unsolved homicides page on the city’s website. Through DNA information and other investigative…
Lire la suite »
Man who killed NH beauty school student Laura Kempton in 1981 is identifiedThe man who killed a beauty school student in Portsmouth, New Hampshire, more than four decades ago has been identified, authorities said Thursday. Laura Kempton, 23, was found dead of massive head trauma at an apartment on Chapel Street on Monday, Sept. 28, 1981, according to an unsolved homicides page on the city’s website. Through DNA information and other investigative…
Lire la suite »
Overexpression of POLA2 in hepatocellular carcinoma is involved in immune infiltration and predicts a poor prognosis - Cancer Cell InternationalBackground Hepatocellular carcinoma (HCC) is the second malignancy worldwide. POLA2 initiates DNA replication, regulates cell cycle and gene repair that promote tumorigenesis and disease progression. However, the prognostic and biological function roles of POLA2 in HCC had not been conclusively determined. Methods The expression levels and prognosis role of POLA1 and POLA2 in HCC were analyzed based on TCGA-LIHC database and recruited 24 HCC patients. Gene mutations were analyzed using “maftools” package. POLA2 and immune cells correlations were analyzed by TIMER. POLA2 co-expressed genes functional enrichment were evaluated using Metascape. The mRNA and protein level of POLA2 was detected in HCC cells and tissues. Cell migration, invasion, proliferation, cell cycle and HCC cell lines derived xenograft model were performed to investigate POLA2 biological function. Results POLA2 was significantly high expressed in HCC than in normal liver tissue in both TCGA-LIHC and our collected HCC samples. In validation cohort, POLA2 significantly related to tumor differentiation, tumor size and Ki-67 (p | 0.05). In TCGA-LIHC cohort, overexpression of POLA2 predicted a low OS and associated with different clinical stages. Multivariate Cox regression showed overexpression of POLA2 effectively distinguished the prognosis at different T, N, M, stages and grades of HCC. POLA2 expression correlated with mutation burden, immune cells infiltration and immune-associated genes expression of HCC. Functional enrichment revealed that POLA2 co-expressed genes were linked to cellular activity, plasma membrane protein complex and leukocyte activity, immune response-regulated cell surface receptor signaling pathway, and immune response-regulated signaling pathway. Moreover, POLA2 was also positively co-expressed with some immune checkpoints (CD274, CTL-4, HAVCR2, PDCD1, PDCD1LG2, TIGIT, and LAG3) (p | 0.001). Gene knockdown revealed that POLA2 promoted proliferation, migration, invasion, and cel
Lire la suite »
Kevin Spacey trial: Actor was 'tried by social media', court hearsThe defence for the American actor summed up their arguments for the jury on Thursday.
Lire la suite »
Ronaldinho tried to beat Seaman with another free-kick 13 years after legendary strikeTake yourself back to 2002. Britney Spears, MTV and no social media. And, of course, the World Cup. England fancied their chances going to the tournament in South Korea and Japan, taking a phenomen…
Lire la suite »