Innovative precision oncology enabled by micro-organospheres derived from patients 3DCulture CellCulture Oncology TissueCulture precisiononcology microfluidics tumorsphere CellStemCell
By Dr. Priyom Bose, Ph.D.Dec 6 2022Reviewed by Benedette Cuffari, M.Sc. Several patient-derived models of cancer , such as patient-derived organoids and patient-derived xenografts , have been developed to assess the clinical efficacy of new cancer therapies. The success of precision oncology depends on these models, as they capture molecular, morphological, and functional characteristics of a cancer patient’s tumor, which enables accurate prediction of drug response and resistance.
Background Genetics & Genomics eBook Compilation of the top interviews, articles, and news in the last year. Download a free copy The application of PDMC in clinical decision-making processes is challenging, partly because of the technical limitations within each model. For example, patient-derived cell lines undergo genetic and morphological changes over time, thus making them inappropriate for clinical screening.
Due to broad-ranging drug screenings, clinicians have detected the associations between genetic mutations and sensitivity to targeted therapies. Therefore, this model has been used as a potential functional precision medicine technology for making appropriate treatment decisions. However, a slow operational process makes it inefficient for clinical use.
The main working principle behind this development involved adding suspended cells from primary tissues to a three-dimensional extracellular matrix. Subsequently, the cells were mixed with a biphasic liquid to generate microfluidic-based droplet micro-organospheres . Finally, the newly generated MOS were demulsified to remove excess oil and were subsequently cultured as suspension droplets.
This newly developed device supports a wide range of temperatures, including between the 4 °C sample and 37 °C collection blocks. The chamber height of the device is designed to allow for the generation of MOS of an average of 250-450 mm in diameter. These dimensions are appropriate for several cell numbers and sizes.
The size and number of tumorspheres increased with the seeding density per droplet. Interestingly, MOS generated from clinical CRC biopsies exhibited various morphologies.
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