Interleukin-37 production correlates with decrease in the immune response against Epstein-Barr virus in acute infectious mononucleosis, along with decreased liver inflammation, finds a study published in the Italian Journal of Pediatrics.
NKT cells were defined through antibodies that were applied for staining cells. We employed isotype-matched Ab controls in every procedure pursuant to the manufacturer’s guidelines for each staining. We subsequently detected and analyzed the labeled cells using a FACS Canto II flow cytometer and Diva software , respectively.As per the guidelines provided by the manufacturer, we performed an amplification of a 191 bp region of thegene using a commercial real-time PCR kit .
According to reports, EBV mainly attacks B-cells and permanently lodges itself as a dormant infection in resting memory B-cells. After the virus has replicated, numerous antigens are produced on the surface of the cell, which triggers a powerful cytotoxic response mediated primarily by T cells [T cells was significantly much higher in patients with IM while the frequency of CD3-CD19B cells was significantly lower, which is in line with previous studies.
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