Microfluidic-based dynamic BH3 profiling predicts anticancer treatment efficacy - npj Precision Oncology

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Microfluidic-based dynamic BH3 profiling predicts anticancer treatment efficacy - npj Precision Oncology
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Researchers design a microfluidics physics-based device to predict cancer therapy response UniBarcelona nresearchnews

A solution containing 10 mg/mL of bovine serum albumin and 10 µL/mL of blue food dye in MiliQ water was perfused through the first inlet of the chip and only MiliQ water through the second inlet at 200 mbar using a Precision Pressure Control System P2CS pump . Liquid coming out from the outlets was collected in Eppendorfs.

In the case of the primary sample, cells were previously incubated with HBSS buffer , 2% of FBS, and 1:10 of anti-cKit Alexa Fluor 647 antibody for 30 min in ice. 35 µL of the cell suspension was added to every well and the chip was incubated at 37 °C for 16 h. After the incubation, the wells were refilled with complete media and sealed with a glass slide secured with plastic alligator clips.

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