New study offers hope for sensory recovery in patients with lower lip numbness
The present study showed that zfp423 could regulate the transcription of ApoD. The ChIP-Seq and luciferase report analysis showed that the binding motifs and were the functional motifs of Zfp423, mediating ApoD expression regulation. Moreover, in Zfp423-silenced RSC96, the inhibitory effect of exogenous TGF-β on ApoD expression decreased. Therefore, the TGF-β signaling pathway might regulate ApoD expression in Schwann cells through Zfp423.
For some completely recovered patients who needed additional follow-ups for implant treatments, we carried out nerve block anesthesia; 0.3–0.5 mL of Articaine hydrochloride and epinephrine tartrate injection was used to anesthetize the ipsilateral MN, contralateral MN, and ipsilateral buccal nerve to obtain an appropriate anesthetic effect. A pinprick test was performed following each anesthesia to confirm the numb area.
Five rats in each group were measured before and every four days after the operation. The average of five values of each rat was taken on the same day, and two-way ANOVA was used to analyze the postoperative time and the overall difference between the groups; the Dunnett-t test was used to analyze the differences of five means between groups on the same day.Groups of 8‒10-week-old Thy1-YFP 16Jrs/J) mice were purchased from the Jackson Laboratory and crossed in-house.
The whole lower lip was immersed in 4% PFA at room temperature overnight. Next, 25% N, N, N′, N′-Tetrakis ethylenediamine in ddHO solution was applied as the decolorizing reagent. Both the volume ratio and weight ratio are acceptable for this process. The samples were placed in 25% Quadrol for one day at a 37° shaker. Next, tert-Butanol was used for dehydration and degreasing, which contributed to better protection for YFP fluorescence.
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