A study published in BMCMedicine suggests that the VRK serine/threonine kinase 2 gene (VRK2) is associated with a higher risk of depression in humans and is likely to be involved in the pathogenesis of depression.
] previously examined mRNA expression in the peripheral venous blood of remitted depression or current depression and control subjects with no lifetime history of depression or anxiety disorders , using Affymetrix U219 arrays and the GeneTitan System. For differential expression analysis between depression patients and controls,value and effect size were computed for the associations between depression and gene expression based on a linear model consisting of empirical covariates.
Recombinant plasmids were co-transfected with packaging plasmids into HEK293T cells by linear polyethylenimine 40 kDa []. Seventy-two hours post-transfection, cells were collected, resuspended and subjected to 4 freeze–thaw cycles in liquid nitrogen and 37 °C water bath to release AAV virus. Benzonase treatment was used to remove extraviral DNA contamination at 37 °C for 30 min and the reaction mixture was centrifuged at 8000 ×for 90 min at 4 °C.
The murine behavioral data were analyzed using GraphPad Prism software and were presented as mean ± SD. For normally distributed data evaluated by Shapiro–Wilk test, two-tailed-test was used to assess the difference between two groups, and one-way analysis of variance was performed for more than two groups.
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