Researchers Hope to Reduce the Cost of Protein Purification ACS_AMI MIT
By Dr. Priyom Bose, Ph.D.Mar 7 2023Reviewed by Megan Craig, M.Sc. The most expensive step in the manufacturing of protein-based drugs is the purification phase. This step involves isolating protein from the bioreactor used for its production, accounting for almost half of the total cost of protein-based drug development. Recently, a team of MIT engineers devised an innovative method of purification that can significantly reduce the cost of the purification step.
A Novel Nano-based Method Designed for Protein Purification MIT engineers sought to address these problems to reduce the production cost of the protein purification process. Professor Kripa Varanasi of MIT and the senior author of this study stated, “The goal is to reduce the cost so that this kind of drug manufacturing becomes affordable in the developing world."
The current approach focussed on binding proteins to specific sites using a templated architecture. Varanasi explained, “This work uses bioconjugate-functionalized nanoparticles to act as templates for enhancing protein crystal formation at low concentrations,". Put simply: nanoparticles were functionalized such that they could selectively form covalent bonds to specific amino acids.
The newly developed strategy was characterized using three crystallization experiments: batch crystallization, vapor diffusion, and droplet crystallization. The size, quality, and quantity of protein crystals were assessed using the vapor diffusion method. Batch crystallization helped measure protein crystallization possibility across a wide range of salt and protein concentrations. The nucleation rate and crystallization induction times were evaluated via the droplet crystallization approach.
Caroline McCue, an MIT graduate student and lead author of the study, stated, "Even at low protein concentrations, we see a lot more crystals forming with these bioconjugate-functionalized nanoparticles.”
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