Scientists develop a pseudovirus system to deep mutational scan the full SARS-CoV-2 spike Coronavirus Disease COVID GenomicSequencing SARSCoV2 jbloom_lab biorxivpreprint
By Dr. Liji Thomas, MDOct 18 2022Reviewed by Benedette Cuffari, M.Sc. The emergence of the severe acute respiratory syndrome coronavirus 2 triggered the ongoing coronavirus disease 2019 . Both immunologically and biologically, the SARS-CoV-2 spike protein represents an attractive target for researchers looking to counteract or neutralize this virus.
Until the current study, DMS was primarily used to explore mutations on the receptor binding domain of the spike protein, or a small number of mutations on the spike, using cell surface display systems on yeast or mammalian cells. However, this approach limits the ability of DMS to measure neutralization effects accurately; therefore, only antibody binding results are assessed, which are often less clinically relevant.
The researchers selected only mutations that seemed probable during natural viral evolution. These mutations were obtained come from the Global Initiative for Sharing Avian Influenza Data database, thus likely representing the functional spike protein. Each of these mutations received its own barcode after integrating the pseudovirus into its final host cell.
What did the study show? A test case using a set of BA.1 neutralizing antibodies targeting the RBD , NTD , and S2 was then conducted. [This] emphasizes the importance of directly mapping escape mutations in SARS-CoV-2 in addition to assessing breadth across other natural SARS-related coronaviruses.”
Belgique Dernières Nouvelles, Belgique Actualités
Similar News:Vous pouvez également lire des articles d'actualité similaires à celui-ci que nous avons collectés auprès d'autres sources d'information.
Hesitancy for receiving regular SARS-CoV-2 vaccination in UK healthcare workers: a cross-sectional analysis from the UK-REACH study - BMC MedicineBackground Regular vaccination against SARS-CoV-2 may be needed to maintain immunity in ‘at-risk’ populations, which include healthcare workers (HCWs). However, little is known about the proportion of HCWs who might be hesitant about receiving a hypothetical regular SARS-CoV-2 vaccination or the factors associated with this hesitancy. Methods Cross-sectional analysis of questionnaire data collected as part of UK-REACH, a nationwide, longitudinal cohort study of HCWs. The outcome measure was binary, either a participant indicated they would definitely accept regular SARS-CoV-2 vaccination if recommended or they indicated some degree of hesitancy regarding acceptance (probably accept or less likely). We used logistic regression to identify factors associated with hesitancy for receiving regular vaccination. Results A total of 5454 HCWs were included in the analysed cohort, 23.5% of whom were hesitant about regular SARS-CoV-2 vaccination. Black HCWs were more likely to be hesitant than White HCWs (aOR 2.60, 95%CI 1.80–3.72) as were those who reported a previous episode of COVID-19 (1.33, 1.13–1.57 [vs those who tested negative]). Those who received influenza vaccination in the previous two seasons were over five times less likely to report hesitancy for regular SARS-CoV-2 vaccination than those not vaccinated against influenza in either season (0.18, 0.14–0.21). HCWs who trusted official sources of vaccine information (such as NHS or government adverts or websites) were less likely to report hesitancy for a regular vaccination programme. Those who had been exposed to information advocating against vaccination from friends and family were more likely to be hesitant. Conclusions In this study, nearly a quarter of UK HCWs were hesitant about receiving a regular SARS-CoV-2 vaccination. We have identified key factors associated with hesitancy for regular SARS-CoV-2 vaccination, which can be used to identify groups of HCWs at the highest risk of vaccine hesitancy and tailor
Lire la suite »
Antimicrobial coating reduces spread of SARS-CoV-2 on public transportAntimicrobial coating reduces spread of SARS-CoV-2 on public transport Coronavirus Disease COVID SARSCoV2 Coating Antimicrobial PublicTransport biorxivpreprint UKHSA
Lire la suite »
Using traveler arrival COVID-19 screening data for real-time SARS-CoV-2 surveillanceIn a new study, researchers developed a model to reconstruct the estimates of how many travelers would have tested severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-positive at the country of departure.
Lire la suite »
Researchers assess wastewater sampling for SARS-CoV-2 surveillanceResearchers assessed the feasibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) surveillance using wastewater sampling.
Lire la suite »
Reduced initiation and duration of breastfeeding in SARS-CoV-2-positive mothersA recent study published in the journal Academic Pediatrics evaluated the associations between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and early breastfeeding.
Lire la suite »
Did the diagnosis and management of Hodgkin lymphoma differ during the SARS-CoV-2 pandemic?In a new study, researchers evaluated the impact of the SARS-CoV-2 pandemic on the diagnosis and treatment of classical Hodgkin lymphoma (cHL) patients in Istanbul, Turkey.
Lire la suite »