Plastic particles themselves, not just chemical additives, can alter sexhormones RutgersU
was chosen for the model to reflect the average exposure concentration in the experimental animal chamber. The aerosol exposure parameters input into the model were based on real-time monitoring and time-integrated sampling data: Count median diameter = 2.81 μm, GSD = 1.3 and Density of 1.14 g/cm]. The following rat lung and breathing parameters were used in the model: Symmetric Sprague Dawley Rat; Body weight = 233 g; Breathing Frequency: 124.6 #/min; TLC = 11.59 mL; FRC = 3.
Lungs were excised and examined for any gross morphological changes. Lungs were perfused with 3% paraformaldehyde in PBS, fixed in 3% paraformaldehyde for 12 h, and then transferred to 50% ethanol. Tissues were embedded in paraffin wax and sectioned. Paraffin-embedded tissues were sectioned at 5 μm, stained with hematoxylin and eosin, and examined by a board-certified veterinary pathologist via light microscopy.
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