A complete map of all the connections in an entire mammalian brain may be in sight. Allen Institute researchers have just launched three new projects to construct large, detailed maps of neuronal connections in sections of the mouse and macaque brains, with an eye toward creating full wiring diagrams of these animals' brains in the future.
Reviewed by Danielle Ellis, B.Sc.Sep 26 2023 These projects are funded by the National Institutes of Health's Brain Research Through Advancing Innovative Neurotechnologies® Initiative.Project 1: Enhancing transmission electron microscopy techniques to visualize brain cell shape and cell-to-cell connection networks of the mouse brain
Transmission electron microscopy is a technique in which a beam of electrons is shot through a tissue sample to create an extremely detailed image.
It works by tagging each cell with a unique RNA barcode that makes it stand out in a cell population. By "connecting the dots" between each barcode, you can trace where and how far a brain cell-;namely its axons and dendrites-;extend. We can actually map the whole macaque brain in a few years instead of 100 years. This is the main motivation." Related StoriesProject 3: Enhancing a Patch-seq pipeline to yield more data, faster results, and linking different datasets to uncover form and function in the whole mouse brain
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