A study published in Cancer Cell International finds that tumor-derived small extracellular vesicles promote breast cancer progression by upregulating PD-L1 expression in macrophages.
In this study, for the first time, the alteration and functions of TAMs in metastasis BC were explored. Further detailed mechanistic experiments revealed that BC-derived sEV-miR-106b-5p and sEV-miR-18a-5p synergistically can promote the PD-L1 expression in M2 TAMs by regulating the PTEN/AKT and PIAS3/STAT3 pathways, contributing to BC metastasis, suggesting a new target for BC immunotherapy.
CD14 + monocytes were isolated from human peripheral blood mononuclear cell through bead separation technology. The macrophages were induced by stimulation of CD14 + monocyte with 50 ng/mL M-CSF or THP-1 with 100 ng/mL of PMA and they were cultured in RPMI-1640 with 10% of FBS at 37 °C with 5% CO2.To analyze the different cells interaction influence, a co-culture system was established using transwell chambers .