Taking a 3D view of the genome may help treat pediatric brain cancers sbpdiscovery NatureComms
). To strengthen the specificity additional information was included to mark genes that were previously verified to be PFA specific via CRISPR-Cas vs fNSC and GBM cell lines.For the visualization of selected genomic loci via Integrative Genomics Viewer, BAM files from samples of the same ependymoma group were merged using samtools and further converted into bigWig files using UCSC tools.
. GBM2 cells were grown in a mixed DMEM/F12 and Neurobasal medium supplemented with following components 1 M of Hepes, Sodium pyruvate, MEM, PDGF-AA growth factor in addition to 1 μg/ml of Heparin , 2mM-Glutamine, B27 without Vitamine A and 20 ng/ml of EGF and FGF-basic. All cells were routinely tested for mycoplasma contamination and authenticated by Single Nucleotide Polymorphism profiling . All cell models were grown at 37 °C with 5% CO2.
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