A study published in the Journal of Translational Medicine finds that Icariin can improve Alzheimer’s disease-induced cognitive impairment and may serve as a potential therapeutic compound to prevent AD development via the microbiome–metabolite–brain axis.
]. Together, gut microbiota analysis, metabolomics, and NP we can be used to conduct in-depth research on the anti-AD effects of ICA.
In this study, the Morris Water Maze test and hematoxylin–eosin staining were used to assess the impact of ICA on cognitive performance and histopathological changes in APP/PS1 mice. Additionally, 16 s rRNA analysis was used to examine the changes in the gut microbiota of APP/PS1 mice, and liquid chromatography–mass spectrometry -based untargeted metabolomics was used to determine the regulatory effects of ICA on the metabolic profiles of the serum and fecal samples of APP/PS1 mice.
]. The sections were then HE stained and sealed with neutral gum. Finally, the sections were observed and photographed under an optical microscope.A minimum of 5 fecal pellets were retrieved from the rectum of each mouse , placed in a sterile conical tube, and stored at − 80 °C until further analysis.]. Briefly, the samples were subjected to polymerase chain reaction amplification, after which the amplicons were purified and subjected to paired-end sequencing and the raw data was evaluated.
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