Describing the genes associated with the sixthsense NatureComms
) at p1. The libraries were sequenced on an Illumina NextSeq500 platform with high-output flow cells by the Next Generation Sequencing Core Facility of the Max-Delbrück Center for Molecular Medicine.For both datasets we used the scruff v1.4.0 package to demultiplex, map, and generate count matrices. Then, we evaluated each dataset statistics using Scater v1.14.6R package.
Sections were dried, fixed with ice-cold 4% PFA in PBS for 15 min and dehydrated in a series of 50%, 70% and 100% ethanol for 5 min each. Afterwards, sections were treated with hydrogen peroxide solution for 15 min at RT to block endogenous peroxidase activity followed by another wash with 100% ethanol for 5 min. Next either Protease IV or Protease III was applied for 30 min at RT.
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