Overview of current methods, technologies, and applications in the molecular diagnosis of mpox medrxivpreprint ufmg StudentsNCL mpox virus diagnosis moleculardiagnosis diagnostics
By Pooja Toshniwal PahariaDec 13 2022Reviewed by Danielle Ellis, B.Sc. In a recent review posted to the medRxiv* preprint server, researchers provided an overview of existing molecular methods for mpox virus detection.
Data were retrieved from databases such as Science Direct, PubMed, Scielo, Scopus, and VHL databases, in September 2022, of relevant articles from the period between 2012 and 2022. Only full-text articles, including polymerase chain reaction , real-time polymerase chain reaction , and PCR-enzyme linked immunosorbent assays , were analyzed. Case reports, editorial letters, and editorials were excluded, and duplicate articles were removed.
Further, multiplexed real-time PCR assays can be performed for simultaneous detection and differentiation of MPXV and varicella zoster virus with high specificity, sensitivity, and reproducibility, by targeting the B6R gene to yield results with low detection limits. PCR-ELISA is a combination of PCR and ELISA techniques and utilizes ELISA as an alternative to the gel electrophoresis step. ELISA is an analytical and environment-friendly technique used for identifying antigen-antibody reactions based on the binding capacities and color changes observed after enzyme-substrate reactions. These are performed to identify and quantify specific molecules. MPXV DNA is labeled with biotin or digoxigenin and can be identified based on color changes.
Another method is the RPA amplification, a modification of the isothermal amplification technique, utilizing DNA polymerase, allowing thermally stable reactions . The test is reportedly 100% specific and 95% sensitive and yields results within 10 minutes.
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