Airpollution shown to worsen movement disorder after stroke Hiroshima_Univ
]. Anesthesia was initially achieved with isoflurane at 3.5% and then reduced to 2% during the surgical procedure. Mice were placed in a stereotaxic frame, and then the scalp was incised. Rose Bengal was injected intraperitoneally. The LED was illuminated for 20 min, positioned posterior 2 mm and left 2 mm relative to Bregma . Decreases in blood flow were determined by laser Doppler flowmetry , and mice with a 70 to 85% decrease in blood flow were used for subsequent experiments.
This procedure was repeated twice. Then, one milliliter of acetone was added to the resulting pellet. The mixture was vortexed for 5 min and then centrifuged at 30,000 × g for 5 min. This procedure was repeated twice. Finally, one milliliter of dichloromethane was added to the resulting pellet. The mixture was vortexed for 5 min and then centrifuged at 30,000 × g for 5 min. This procedure was repeated twice. The pellet was dried overnight in a desiccator.
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