In this article, Roche outlines a novel method by which one can streamline their KAPA RNA workflowsthrough the utilization of ribodepletion.
Sponsored Content by Roche Sequencing and Life ScienceAug 22 2023Reviewed by Alex Smith Since the emergence of RNA sequencing, laboratories and sequencing organizations have faced a significant challenge in terms of the extensive time required for ribosomal RNA depletion and RNA library preparation.
As a result, a more streamlined and efficient process is achieved, delivering high-quality, ribodepleted libraries and sequencing metrics while reducing reagent and plastic consumption. Figure 2. The % aligned reads is similar with both workflows. Image Credit: Roche Sequencing and Life ScienceFigure 3. The % of reads that represent residual rRNA remains very low with both workflows. Image Credit: Roche Sequencing and Life ScienceFigure 4. The %GC content and GC distribution metrics are similar with both workflows. %GC content for all four input samples. GC distribution for all four input samples.
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