I opened the Quality Street, Heroes, Celebrations & Roses to see what’s in each one - and there were a few surprises
With 11 days to go until Christmas, now seems as good of a time as any to FINALLY open up those Christmas chocolate tins and scoff all the best ones. We do usually wait until after my birthday on December 22 to open them - but I took one for the team in the name of research, this year.
Some of the chocolates are no longer being wrapped in shiny packaging, as Nestle moves to a more sustainable wrapper. Toffee pennies were next with just six of those, matching the number of orange creme, while there were five Purple Ones, coconut eclairs and orange chocolate crunches. Bounty fans would enjoy no less than eight coconut chocolates, matching the nation’s favourite Malteasers, which is said to be the most searched for, according to Britsuperstore.
Mondelez, who owns Cadbury, explained: "To ensure we can continue to bring our loyal consumers the products they know and love, we have temporarily substituted bitesize Cadbury Twirl in Heroes Tubs, to the slightly larger Cadbury Twirl 21g. "Rest assured consumers can enjoy the same amount of chocolate as before with the overall weight of the product remaining the same.”
Belgique Dernières Nouvelles, Belgique Actualités
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Nanopore long-read-only metagenomics enables complete and high-quality genome reconstruction from mock and complex metagenomes - MicrobiomeBackground The accurate and comprehensive analyses of genome-resolved metagenomics largely depend on the reconstruction of reference-quality (complete and high-quality) genomes from diverse microbiomes. Closing gaps in draft genomes have been approaching with the inclusion of Nanopore long reads; however, genome quality improvement requires extensive and time-consuming high-accuracy short-read polishing. Results Here, we introduce NanoPhase, an open-source tool to reconstruct reference-quality genomes from complex metagenomes using only Nanopore long reads. Using Kit 9 and Q20+ chemistries, we first evaluated the feasibility of NanoPhase using a ZymoBIOMICS gut microbiome standard (including 21 strains), then sequenced the complex activated sludge microbiome and reconstructed 275 MAGs with median completeness of ~ 90%. As a result, NanoPhase improved the MAG contiguity (median MAG N50: 735 Kb, 44-86X compared to conventional short-read-based methods) while maintaining high accuracy, allowing for a full and accurate investigation of target microbiomes. Additionally, leveraging these high-contiguity reference-quality genomes, we identified 165 prophages within 111 MAGs, with 5 as active prophages, indicating the prophage was a neglected source of genetic diversity within microbial populations and influencer in shaping microbial composition in the activated sludge microbiome. Conclusions Our results demonstrated that NanoPhase enables reference-quality genome reconstruction from complex metagenomes directly using only Nanopore long reads. Furthermore, besides the 16S rRNA genes and biosynthetic gene clusters, the generated high-accuracy and high-contiguity MAGs improved the host identification of critical mobile genetic elements, e.g., prophage, serving as a genomic blueprint to investigate the microbial potential and ecology in the activated sludge ecosystem. Video Abstract
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