Researchers identify unique pain pathway from australian stinging tree for non-opioid pain relief uq_news NatureComms
-targeting toxins appear to bind to the α subunit, making the gympietides toxins that require an interacting protein to modulate Nachannel function. In addition, the gympietides are also dispanin ligands, raising the possibility of future development of selective ligands that may find use as tool compounds to interrogate the function of this protein family, or that may ultimately lead to therapeutic applications. Finally, our study reports an association with Na1.
Preparative and analytical HPLC were carried out on a Shimadzu LC-20AT system equipped with SPD-20A Prominence UV/VIS detector, an SIL-20AHT auto-injector. An Eclipse XDB – C18 column with gradient from 45–75% solvent B in 60 min was used to purify the oxidized peptides. A Zorbax 300SB – C18 column with gradient from 45–75% solvent B in 60 min was used to purify tagged peptides.
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